A structured single-cell workflow for cell-level transcriptomic analysis and interpretation.
6.1 System Path
Code
flowchart TD A[Raw Counts] B[Cell and Gene QC] C[Normalization] D[Dimensional Reduction] E[Clustering] F[Marker Detection] G[Cell Annotation] H[Biological Interpretation] I[Reporting] A --> B B --> C C --> D D --> E E --> F F --> G G --> H H --> I style A fill:#dbeafe,stroke:#2563eb,stroke-width:2px,color:#0f172a style B fill:#e0f2fe,stroke:#0284c7,stroke-width:2px,color:#0f172a style C fill:#ecfeff,stroke:#0891b2,stroke-width:2px,color:#0f172a style D fill:#ede9fe,stroke:#7c3aed,stroke-width:2px,color:#0f172a style E fill:#f3e8ff,stroke:#9333ea,stroke-width:2px,color:#0f172a style F fill:#fae8ff,stroke:#c026d3,stroke-width:2px,color:#0f172a style G fill:#fef3c7,stroke:#d97706,stroke-width:2px,color:#0f172a style H fill:#ecfccb,stroke:#65a30d,stroke-width:2px,color:#0f172a style I fill:#f0fdf4,stroke:#16a34a,stroke-width:2px,color:#0f172a
flowchart TD
A[Raw Counts]
B[Cell and Gene QC]
C[Normalization]
D[Dimensional Reduction]
E[Clustering]
F[Marker Detection]
G[Cell Annotation]
H[Biological Interpretation]
I[Reporting]
A --> B
B --> C
C --> D
D --> E
E --> F
F --> G
G --> H
H --> I
style A fill:#dbeafe,stroke:#2563eb,stroke-width:2px,color:#0f172a
style B fill:#e0f2fe,stroke:#0284c7,stroke-width:2px,color:#0f172a
style C fill:#ecfeff,stroke:#0891b2,stroke-width:2px,color:#0f172a
style D fill:#ede9fe,stroke:#7c3aed,stroke-width:2px,color:#0f172a
style E fill:#f3e8ff,stroke:#9333ea,stroke-width:2px,color:#0f172a
style F fill:#fae8ff,stroke:#c026d3,stroke-width:2px,color:#0f172a
style G fill:#fef3c7,stroke:#d97706,stroke-width:2px,color:#0f172a
style H fill:#ecfccb,stroke:#65a30d,stroke-width:2px,color:#0f172a
style I fill:#f0fdf4,stroke:#16a34a,stroke-width:2px,color:#0f172a